ABC transporters and azole susceptibility in laboratory strains of the wheat pathogen Mycosphearella graminicola

Laboratory strains of Mycosphaerella graminicola with decreased susceptibilities to the azole antifungal agent cyproconazole showed a multidrug resistance phenotype by exhibiting cross-resistance to an unrelated chemical, cycloheximide or rhodamine 6G, or both. Decreased azole susceptibility was found to be associated with either decreased or increased levels of accumulation of cyproconazole. No specific relationship could be observed between azole susceptibility and the expression of ATP-binding cassette (ABC) transporter genes MgAtr1 to MgAtr5 and the sterol P450 14-demethylase gene, CYP51. ABC transporter MgAtr1 was identified as a determinant in azole susceptibility since heterologous expression of the protein reduced the azole susceptibility of Saccharomyces cerevisiae and disruption of MgAtr1 in one specific M. graminicola laboratory strain with constitutive MgAtr1 overexpression restored the level of susceptibility to cyproconazole to wild-type levels. However, the level of accumulation in the mutant with an MgAtr1 disruption did not revert to the wild-type level. We propose that variations in azole susceptibility in laboratory strains of M. graminicola are mediated by multiple mechanisms

A prospective study on obesity and subcutaneous fat patterning in relation to breast cancer in post-menopausal women participating in the DOM project.

The associations of body fat and body fat distribution with breast cancer risk were examined in a prospective study in 9,746 post-menopausal women with a natural menopause, aged 49-66 at intake, participating in a breast cancer screening project (the DOM project in Utrecht). During a follow-up period of 15 years (mean follow-up time 12.5 years) 260 women developed breast cancer. Fat distribution, assessed by contrasting groups of subcapsular and triceps skinfold thickness, was found to be unrelated to breast cancer incidence. No significant relationship between body fat, measured either by weight, Quetelet's index, triceps skinfold or subscapular skinfold, and breast cancer risk was found when analysed in quartiles. However, women in the upper decile compared with the lower decile of the distribution of Quetelet's index were found to have a 1.9 times (95% CI 1.1-3.3) higher risk for breast cancer. These results seemed to be in contrast with the significant positive association between fatness, analysed in quartiles, and breast cancer observed in a cross-sectional study, based on mammographic screening, carried out previously in the same population. Although the differences between the present, prospective, study and our cross-sectional study may be due to chance it may be that there are differences between characteristics of breast cancer detected at screening and subsequently, which influence the associations between measures of fatness and risk of breast cancer

What tweets tell us about MOOC participation

In this research paper, the authors analyze the collected Twitter data output during MobiMOOC 2011. This six-week data stream includes all tweets that contain the MOOC's hashtag (#mobiMOOC) and it has been analyzed using qualitative methodology. The analysis sought to examine the emotive vocabulary used, to determine if there was content-sharing via tweets, and to analyze the folksonomic trends of the tweets. In Addition sought a deeper understanding of what, and how, MOOC participants share what they share on the MOOC's Twitter channel. The aim of this study is to provide a little more insight into MOOC learner behaviors on Twitter so that future MOOC designers and facilitators can better engage with their learners.Facultad de Ciencias Exacta

What tweets tell us about MOOC participation

In this research paper, the authors analyze the collected Twitter data output during MobiMOOC 2011. This six-week data stream includes all tweets that contain the MOOC's hashtag (#mobiMOOC) and it has been analyzed using qualitative methodology. The analysis sought to examine the emotive vocabulary used, to determine if there was content-sharing via tweets, and to analyze the folksonomic trends of the tweets. In Addition sought a deeper understanding of what, and how, MOOC participants share what they share on the MOOC's Twitter channel. The aim of this study is to provide a little more insight into MOOC learner behaviors on Twitter so that future MOOC designers and facilitators can better engage with their learners.Facultad de Ciencias Exacta

Raising awareness of the accessibility challenges in mathematics MOOCs

MOOCs provide learning environments that make it easier for learners to study from anywhere, at their own pace and with open access to content. This has revolutionised the field of eLearning, but accessibility continues to be a problem, even more so if we include the complexity of the STEM disciplines which have their own specific characteristics. This work presents an analysis of the accessibility of several MOOC platforms which provide courses in mathematics. We attempt to visualise the main web accessibility problems and challenges that disabled learners could face in taking these types of courses, both in general and specifically in the context of the subject of mathematics

Challenges while MOOCifying a HE eLearning course on Universal Accessibility

There are some similarities in developing a traditional Higher Education (HE) eLearning course and MOOCs (Massive Open Online Courses), due to the use of the basis of eLearning instructional design. But in MOOCs, students should be continually influenced by information, social interactions and experiences forcing the faculty to come up with new approaches and ideas to develop a really engaging course. In this paper, the process of MOOCifying an online course on Universal Accessibility is detailed. The needed quality model is based upon the one used for all online degree programs at our university and on a variable metric specially designed for UNED MOOC courses making possible to control how each course was structured, what kind of resources were used and how activities, interaction and assessment were included. The learning activities were completely adapted, along with the content itself and the on-line assessment. For this purpose, the Gardner's Multiple Intelligences Product Grid has been selected

Exploring Large Document Repositories with RDF Technology: The DOPE Project

This thesaurus-based search system uses automatic indexing, RDF-based querying, and concept-based visualization of results to support exploration of large online document repositories